What is Streak Plate Method ? Striking (Streak Plate Method) is a microbiology technique for isolating a pure strain from a single species of microbe, most commonly bacteria. Loeffler and Gaffky invented the dilution or isolation by streaking method in Koch’s laboratory, which includes systematically streaking bacteria across the exterior of the agar in a … Read more
What is Bile Solubility Test ? The bile solubility test is a biochemical test used to distinguish Streptococcus pneumoniae from other alpha-hemolytic Streptococci and to confirm the diagnosis. The bile solubility test has been utilised as a critical test for S. pneumoniae differentiation because it enables for the difficult work of distinguishing between the two … Read more
What is Butyrate Disk Test ? The capacity of Moraxella catarrhalis to use bromochloro-indolyl butyrate or 4-methylumbelliferyl butyrate is used in the butyrate disc test, which is a quick identification test for Moraxella catarrhalis. Otitis media, sinusitis, conjunctivitis, bronchitis, and pneumonia are all caused by Moraxella (Branhamella) catarrhalis. Furthermore, many M. catarrhalis strains develop the … Read more
What is Beta Lactamase Test ? The chromogenic cephalosporinase test is one of the most important tests in clinical laboratories for β – lactamase detection. The substrate is chromogenic cephalosporin, while the test disc is chromogenic cephalosporin. When organisms with β -lactamases are put to the disc, they work by opening the substrate’s β -lactam … Read more
What is Bacitracin Susceptibility Test ? Bacillus licheniformis produces bacitracin, which prevents the synthesis of peptidoglycan, a significant component of the bacterial cell wall. When bacitracin is present, it prevents bactoprenol from transferring NAM and NAG sugars across the cell membrane, preventing peptidoglycan formation. The most common way to distinguish beta hemolytic Group A streptococci … Read more
What is Bile Esculin Test ? The bile esculin test is a biochemical test that uses the capacity to hydrolyzeesculin to distinguish Enterococci and group D Streptococci from non-group D viridans group Streptococci. Although many species can hydrolyzeesculin, only a handful can do it in the presence of bile (4 percent bile salts or 40 … Read more
What is Acetamide Utilization Test ? Acetamide Utilization Test: One of several biochemical assays used to identify aerobic organisms is the acetamide consumption test. The acetamide test is used to assess an organism’s ability to deamidate Acetamide. Based on its usage, Acetamide is used in qualitative processes to differentiate non-fermentative, gram-negative bacteria, primarily Pseudomonas aeruginosa. … Read more
What is MUG Test? MUG Test: MUG stands for 4-Methylumbelliferyl-β-D Glucuronide and it acts as a substrate for the organisms those having enzyme β-glucuronidase. This enzyme is present in 97% strains of E. coli .Organisms other than E. coli of the Enterobacteriaceae family Salmonella, Shigella and Yersinia also possess the enzyme β-glucuronidase. Objectives of MUG … Read more
What is Acetate Utilization Test? The acetate utilization test is a biochemical experiment that evaluates an aerobic organism’s capacity to consume acetate in the growth medium. The acetate consumption test is often used in qualitative approaches to differentiate Shigella species from Escherichia coli and non-fermentative gram-negative bacteria. The ability of an organism to use acetate … Read more
What is Benedict’s Test ? Benedict’s test is a simple chemistry test used to detect reducing sugars. Reducing sugars are carbohydrates having free aldehyde or ketone functional group in its molecular structure. This includes all monosaccharides (eg. glucose, fructose, galactose) and many disaccharides, including lactose and maltose.Benedict’s test is most commonly used to test for … Read more