PhD Nest

Butyrate Disk Test: Objective, Principle, Procedure, Results, Uses, Limitations  

Butyrate Disk Test: Objective, Principle, Procedure, Results, Uses, Limitations  

What is Butyrate Disk Test ?  The capacity of Moraxella catarrhalis to use bromochloro-indolyl butyrate or 4-methylumbelliferyl butyrate is used in the butyrate disc test, which is a quick identification test for Moraxella catarrhalis. Otitis media, sinusitis, conjunctivitis, bronchitis, and pneumonia are all caused by Moraxella (Branhamella) catarrhalis. Furthermore, many M. catarrhalis strains develop the … Read more

Bacitracin Susceptibility Test: Objective, Principle, Procedure, Results, Uses, Limitations

Bacitracin Susceptibility Test: Objective, Principle, Procedure, Results, Uses, Limitations

What is Bacitracin Susceptibility Test ? Bacillus licheniformis produces bacitracin, which prevents the synthesis of peptidoglycan, a significant component of the bacterial cell wall. When bacitracin is present, it prevents bactoprenol from transferring NAM and NAG sugars across the cell membrane, preventing peptidoglycan formation. The most common way to distinguish beta hemolytic Group A streptococci … Read more

Acetamide Utilization Test: Objective, Principle, Procedure, Results, Uses, Limitations

Acetamide Utilization Test: Objective, Principle, Procedure, Results, Uses, Limitations

What is Acetamide Utilization Test ? Acetamide Utilization Test: One of several biochemical assays used to identify aerobic organisms is the acetamide consumption test. The acetamide test is used to assess an organism’s ability to deamidate Acetamide. Based on its usage, Acetamide is used in qualitative processes to differentiate non-fermentative, gram-negative bacteria, primarily Pseudomonas aeruginosa. … Read more

Acetate Utilization Test- Principle, Procedure, Result, Uses, Limitations

Acetate Utilization Test- Principle, Procedure, Result, Uses, Limitations

What is Acetate Utilization Test? The acetate utilization test is a biochemical experiment that evaluates an aerobic organism’s capacity to consume acetate in the growth medium. The acetate consumption test is often used in qualitative approaches to differentiate Shigella species from Escherichia coli and non-fermentative gram-negative bacteria. The ability of an organism to use acetate … Read more

Benedict’s Test: Objectives, Principle, Procedure, Results, Limitations

Benedict’s Test: Objectives, Principle, Procedure, Results, Limitations

What is Benedict’s Test ? Benedict’s test is a simple chemistry test used to detect reducing sugars. Reducing sugars are carbohydrates having free aldehyde or ketone functional group in its molecular structure. This includes all monosaccharides (eg. glucose, fructose, galactose) and many disaccharides, including lactose and maltose.Benedict’s test is most commonly used to test for … Read more

Catalase test: Principle, Procedure, Results, Precautions, Limitations Uses

Catalase test: Principle, Procedure, Results, Precautions, Limitations Uses

What is Catalase Test? Catalase is an enzyme, which is produced by microorganisms that live in oxygenated environments to neutralize toxic forms of oxygen metabolites; H2O2. The catalase enzyme neutralizes the bactericidal effects of hydrogen peroxide and protects them. Anaerobes generally lack the catalase enzyme. This test demonstrate the presence of catalase, an enzyme that … Read more